Fig. 5

Immunohistochemical staining of matrilin-1 in the tracheal and auricular specimen. Immunohistochemical staining of tracheal and auricular cartilage with anti-matrilin-1 antibody. Immunoreactions are visualized with 3, 3-diaminobenzidine and counterstained with hematoxylin. Matrilin-1 immunoreactivity (brown, arrow) is detected mainly in the boundary region of the cartilage and some chondrocytes in the trachea (a, c), but not in the auricular tissue (b). Asterisk represents the cartilage. Magnifying power is (×100) both in (a) and (b). High-magnification image (×200) is shown in (c). Bar, 100 μm