Fig. 1

Clinical and histological anti-inflammatory effects of intermittent fasting in the collagen-induced arthritis model. (A) Experimental design for the evaluation of anti-inflammatory effects of IF and the CIA. (B) Graph showing the monitoring of the incidence of clinical arthritis throughout the experiment (n = 8 mice per group). (C) Graph showing the evaluation of the effect of IF on arthritis severity throughout the experiment (n = 8 mice per group). (D) Graph showing the monitoring of mouse weight throughout the experiment (n = 8 mice per group). (E) Representative images of clinical severity of CIA mice with and without IF. (F) Representative images of the effect of IF on arthritis structural damage through microtomography. (G) Representative images and evaluation of the influence of IF on arthritis histological outcomes using H&E staining (n = 8 mice per group). The arrows indicate the histological area of ​​the finding evaluated in each image. The student t-test was used to determine differences between groups *: p ≤ 0.05; **: p ≤ 0.01; ***: p ≤ 0.001. CIA: Collagen-induced arthritis