Fig. 4

Effect of Intermittent Fasting on the expression of IL-33, BGLAP, HIF-1α, and PPAR-γ in the hind paws’ joints of collagen-induces arthritis mice. Representative images of the expression of IL-33 (A), BGLAP (B), HIF-1α (C), and PPAR-γ (D) in tarsal joints of DBA/1 mice non-treated (left) and treated with IF (right). The images were acquired with 10X and 40X amplification. Optical Density (OD) mean and standard error (SE) are shown in graphics (A’, B,’ C,’ and D’). The student’s t-test was used to determine statistically significant differences. *: p≤0.05, ** p≤0.01. n = 8 mice per group